hct116 puromycin concentration

  • suppresses colony formation ability of human colorectal carcinoma

    May 17, 2012 to silence ALEX1 gene in HCT116 and SW480 cells. Our current mented with lower concentrations of puromycin (1 lg/mL) than that used in 

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  • Why is my transduce cells dying following Puromycin selection?

    I then started Puromycin selection at concentration of 1ug/mL. After 2 days, my Can lentivirus infect cells without polybrene (HCT116, T24, and A549 cells)?.

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  • Characterization of the Loss of SUMO Pathway Function - PLOS ONE

    Conditional SAE2 knockdown in U2OS and HCT116 cells slowed cell growth in vitro, and Luciferase activity was measured at Day 5 post puromycin selection.

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  • A Vulnerability of a Subset of Colon Cancers with - Cell Press

    Apr 7, 2016 (WiDr and HCT116) CC cell lines were infected with the RANBP2 shRNAs #1 or #3 and pLKO as a control. After puromycin selection, cells 

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  • Synthesis and Preclinical Evaluation of a Highly Improved

    Mar 28, 2016 Colon cancer (Caco-2, HT29, HCT116) and normal colon At high concentration , puromycin acts as an inhibitor of protein synthesis. However 

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  • Establishment of a colorectal cancer nude mouse visualization

    Mar 1, 2016 infect CRC SW480, SW620, LoVo and HCT116 cells. Puromycin was used for the selection and large-scale amplifiion of the stable HIF-1α 

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  • PRDX1 and MTH1 cooperate to prevent ROS-mediated inhibition of

    oGen) selection. HCT116 cells expressing TSQ1–hTR were gen- erated by lentiviral transduction and puromycin selection for. 7 d. All cells were cultured in 

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  • Candidate Biomarkers of Response to an Experimental Cancer

    Sep 15, 2009 by transduction of HCT116 cells with the shRNA library. After infection, the cells were selected with puromycin (2 μg/mL). When the selection.

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  • Identifiion of a novel quinoline-based DNA demethylating

    May 6, 2019 Moreover, MC3353 was tested in a panel of cancer cells (HCT116 colon cells, and the stable clone was obtained by puromycin selection.

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  • Single-vector inducible lentiviral RNAi system for oncology target

    Dec 22, 2008 beyond DAOY cells, we transduced HeLa and HCT116 cells with the inducible Following puromycin selection, the resulting stable cell.

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  • Puromycin | Thermo Fisher Scientific - BR

    Puromycin has a fast mode of action, causing rapid cell death at low antibiotic concentrations. Adherent mammalian cells are sensitive to concentrations of 2 to 5 

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  • Supporting Information - PNAS

    HCT116 (a colorectal cancer cell line) and U2OS HCT116 cells stably expressing BUB1B shRNA were generated jected to puromycin selection (5 μg /mL).

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  • Cas9 Expressing HCT116 Cell Line - Applied Biological Materials

    Incubate cultures at 37°C, 5% CO2. Shipping Conditions, Dry Ice. Quality Control, 1) Drug selection by selection marker. Tumorgenic? Yes 

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  • Can any one explain to me why the concentration of G418 is

    For the general info, HCT116-/- cells are inherently resistant to hygromycin and neomycin as Yes one can go for blasticidin or puromycin, i did with blasticidin.

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  • p53-Dependent Apoptotic Effect of Puromycin via Binding of - MDPI

    Apr 24, 2019 HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with 

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  • HCT 116 cells | Cell-culture Database - TOKU-E

    Cells name, Plasmid/Vector, Antibiotic, Concentration, References. HCT 116. peak12. Puromycin. 0.8μg/ml. 322. HCT 116. phMLH1 · pcDNA3.1/Hygro.

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  • Why is my transduce cells dying following Puromycin selection?

    I then started Puromycin selection at concentration of 1ug/mL. After 2 days, my Can lentivirus infect cells without polybrene (HCT116, T24, and A549 cells)?.

    Chat Online
  • Epithelial interleukin-4 receptor expression promotes colon tumor

    Feb 22, 2010 The human colon tumor cell lines HCT116, HT29, DLD-1, SW480, murine or human cells were placed under selection with puromycin (10 

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  • A CRISPR screen reveals a WNT7B-FZD5 signaling circuit - bioRxiv

    and HCT116 colorectal cancer cells (Fig. S2a-b), which may infection (and 48 hours post puromycin selection) cells were split into two independent replie.

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  • HCT116-eGFP-Puro Reporter Gene Cell Line - Imanis Life Sciences

    Species, Human. Cell Type, Colorectal Carcinoma. Transgene, Enhanced green fluorescent protein (eGFP). Selection Gene, Puromycin resistance (Puro) 

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